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* For the first project, during the regular lab period for Lab 6, groups will be given one large flask of cells to subculture into eight culture plates each at 1×105 viable cells/plate, with two replicate plates for each concentration of NGF. This completes day 1. Then on days 2, 3 and 4, exactly 100 cells in two representative fields of view per plate will be observed, and the number out of 100 that have neurite-like outgrowths will be recorded. This project will include medium changes to replenish the NGF on all days but the last. For the second project, groups will be given one or two large flasks of cells to subculture into eight culture plates, but each will contain as high a concentration of viable cells per plate as possible. This completes day 1. On days 2 and 3, NGF will be replenished by changing the medium. On the final day of the project, trypan blue exclusion assays will be done for each plate (in duplicate) to determine the total number of viable cells and the viability percentage for each condition. * no need for materials and method the paper will be ( introduction, Result, Discussion, and reference). * the text portion of the Results section. Always introduce the figure or table in the text of the Results before you show it to your reader. For example, Figure 1 should be inserted as soon as is feasible after noting that: A general trend showing dose-dependent differentiation was observed (Fig.1). The text and the visuals should form a coherent presentation of the data you obtained. When you discuss error bars (and you definitely should), note that whether or not you are able to draw statistical conclusions depends of the number of replicates used. If there are fewer than 6 replicates (2 independent data points from 3 independent experiments), you cannot reliably use error bars to draw conclusions regarding statistical significance. This will be the case for your project, because whereas we will gather 4 data points for each concentration for each point in time, these will not be generated by three independent experiments. Still, you will always be able to examine the error bars to draw conclusions regarding possible trends that could be confirmed or rejected if you were to repeat the experiment enough times, based on the following points: o if two error bars do not overlap, this suggests that more robust data would reveal statistical difference, and vice versa o if an error bar is small (e.g. 10% total) you can conclude that the data is of reasonable quality based on this lack of variation in the samples, and vice versa.

the paper should contain an introduction, result, and discussion where the result should interpret the data in the graphs and the error bar.

 

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